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期刊论文 9

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2022 2

2021 1

2020 1

2018 2

2009 2

2008 1

关键词

互养菌(Syntrophus 1

互营杆菌(Syntrophorhabdaceae) 1

升流式厌氧污泥床(UASB)颗粒污泥 1

苯酚厌氧降解 1

荧光原位杂交 1

隐杆厌氧菌(Cryptanaerobacter 1

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Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the

Bingjie Xu, Gang Xue, Xing Yang

《环境科学与工程前沿(英文)》 2018年 第12卷 第5期 doi: 10.1007/s11783-018-1080-5

摘要:

• An ibuprofen-degrading strain, Serratia marcescens BL1, was isolated and identified.

• The effects of various factors on ibuprofen degradation by BL1 were evaluated.

• Strain BL1 was applied to a laboratory-scale biological aerated filter system.

• Strain BL1 was stable in both static tests and in the biological aerated filter system.

关键词: Ibuprofen     Biological aerated filter     Degrading bacterium     Serratia marcescens    

Characterization of a phenanthrene-degrading methanogenic community

Quanhui Ye, Chengyue Liang, Chongyang Wang, Yun Wang, Hui Wang

《环境科学与工程前沿(英文)》 2018年 第12卷 第5期 doi: 10.1007/s11783-018-1083-2

摘要:

The mixed samples of contaminated soil, sludge and coke wastewater showed great phenanthrene methanogenic degradation potential.

Comamonadaceae, Nocardiaceae and Methanobacterium were dominant members.

Hexane, hexadecane and benzene could enhance phenanthrene degradation.

关键词: Phenanthrene degradation potential     Methanogenic     Bacterial population     Archaeal group    

A heterotrophic nitrification-aerobic denitrification bacterium TJPU05 suitable for nitrogen removal

《环境科学与工程前沿(英文)》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1503-6

摘要:

H. venusta TJPU05 showed excellent HN-AD ability at high salinity.

关键词: Salt-tolerant bacteria     H. venusta TJPU05     Heterotrophic nitrification and aerobic denitrification     High-salinity wastewater    

Studies on culture condition of new marine bacterium sp. SY01

LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua

《化学科学与工程前沿(英文)》 2008年 第2卷 第4期   页码 443-446 doi: 10.1007/s11705-008-0065-5

摘要: New marine bacterium sp. SY01, producer of prodigiosin, was isolated from the seawaters of Sanya Bay. The culture conditions of this bacterium were investigated. sp. SY01 was cultured in 2216E media which contained tryptophan, histidine, lactonic acid, camphor, limonene, casein, diphenyl guanidine, coumarin and 1,3-dinitrobenzene, respectively. After 5 days cultivation, the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode. As the results, tryptophan, histidine and casein didn’t show any observable influences on the biosynthesis of prodigiosin. Lactonic acid, camphor, limonene, diphenyl guanidine, coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a certain extent, slower the culture broth to turn red. However, 1, 3-dinitrobenzene inhibited the bacteria to produce prodigiosin completely. MS data suggested that various metabolites with chemodiversity were produced in different culture media. In particular, a series of high-molecular-weight compounds with high relative abundances were observed in the medium containing limonene. To further optimize the culture condition, more new prodigiosin analogues and lead compounds can be obtained and the goal of “one strain-many compounds” can be achieved.

关键词: producer     high-molecular-weight     relative     culture     different culture    

LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

《环境科学与工程前沿(英文)》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1349-3

摘要: Abstract • Pseudomonas mendocina was first reported for aerobic nitrate removal. • It removed 90% of NO3−-N in 24 h under aerobic conditions. • This strain converted NO3−-N to bio-nitrogen (37.9%) and gaseous nitrogen (49.7%). • Inoculation of this strain increased sludge denitrification rate by 4.3 times. The problem of nitrate accumulation in aerobic tank and total nitrogen excessive discharge in effluent was very common in traditional livestock and poultry farming wastewater treatment systems owing to the lengthy process flow and low process control level. A strain LYX of aerobic bacterium was isolated from the activated sludge of a wastewater treatment system in a pig farm, which could remove nitrate effectively in aerobic tank and was identified Pseudomonas mendocina by 16S rRNA sequencing. Under the condition of nitrate as the sole nitrogen source, this strain removed over 90% of NO3−-N with an initial concentration of 110 mg/L under aerobic conditions within 48 hours. Among them, 37.9% of NO3−-N was assimilated into Bio-N, about 51.9% was reduced to gaseous nitrogen and less than 0.5% of nitrogen was replaced by NO2−-N and NH4+-N, 9.7% NO3−-N remained in the effluent at the end. At the same time, four key genes (napA, nirK, norB and nosZ) related to nitrate nitrogen removal were expressed during the denitrification process of P. mendocina LYX, in which the transcription level of the indicator genes of this aerobic denitrifying bacterium (napA) was the highest. In addition, it was found with the 15N tracer technique that inoculation of this strain on sludge increased the amount of nitrogen loss from 9.26 nmol N/(g·h) to 23.835 nmol N/(g·h). Therefore, P. medocina LYX is a potential bioagent for advanced nitrogen removal by assimilating and reducing nitrate simultaneously in aerobic tanks.

关键词: Pseudomonas mendocina     Aerobic nitrate removal     15N tracing technique     Denitrification assimilatively and disimilatively     Aerobic denitrifying genes    

Pollution and biodegradation of hexabromocyclododecanes: A review

Ling Huang, Syed Bilal Shah, Haiyang Hu, Ping Xu, Hongzhi Tang

《环境科学与工程前沿(英文)》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1190-8

摘要: • Bioremediation is the most cost-effective approach for degradation of HBCDs. • Bacteria or bacterial consortia are used in the cases of bio-augmentation. • Microbes combined with phytoremediation increase the remediation efficiency. Hexabromocyclododecanes (HBCDs) are the most common brominated flame-retardants after polybrominated diphenyl ethers. HBCDs can induce cancer by causing inappropriate antidiuretic hormone syndrome. Environmental contamination with HBCDs has been detected globally, with concentrations ranging from ng to mg. Methods to degrade HBCDs include physicochemical methods, bioremediation, and phytoremediation. The photodegradation of HBCDs using simulated sunlight or ultraviolet lamps, or chemical catalysts are inefficient and expensive, as is physicochemical degradation. Consequently, bioremediation is considered as the most cost-effective and clean approach. To date, five bacterial strains capable of degrading HBCDs have been isolated and identified: Pseudomonas sp. HB01, Bacillus sp. HBCD-sjtu, Achromobacter sp. HBCD-1, Achromobacter sp. HBCD-2, and Pseudomonas aeruginosa HS9. The molecular mechanisms of biodegradation of HBCDs are discussed in this review. New microbial resources should be explored to increase the resource library in order to identify more HBCD-degrading microbes and functional genes. Synthetic biology methods may be exploited to accelerate the biodegradation capability of existing bacteria, including modification of the degrading strains or functional enzymes, and artificial construction of the degradation microflora. The most potentially useful method is combining micro-degradation with physicochemical methods and phytoremediation. For example, exogenous microorganisms might be used to stimulate the adsorption capability of plants for HBCDs, or to utilize an interaction between exogenous microorganisms and rhizosphere microorganisms to form a new rhizosphere microbial community to enhance the biodegradation and absorption of HBCDs.

关键词: Hexabromocyclododecane     Biodegradation     Bioremediation     Phytoremediation     Bacterium    

Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

Longtao ZHANG, Bo JIANG, Wanmeng MU, Tao ZHANG,

《化学科学与工程前沿(英文)》 2009年 第3卷 第4期   页码 393-398 doi: 10.1007/s11705-009-0252-z

摘要: -psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of -psicose from -fructose. In this paper, another bacterium which could convert -fructose to -psicose was isolated and identified as . The process parameters of -psicose production using permeabilized cells of SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10min, and reaction conditions: cell concentration, 30g dry cell wt/L; concentration of substrate, 50g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5g/L -psicose with a -psicose productivity of 0.82g·L·h. This is the first report of bioproduction of -psicose using permeabilized cells of .

关键词: reaction     permeabilized     substrate     bacterium     process    

Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter sphaeroides AR-3

Guanghong ZHENG, Zhuhui KANG, Yifan QIAN, Lei WANG,

《环境科学与工程前沿(英文)》 2009年 第3卷 第4期   页码 387-392 doi: 10.1007/s11783-009-0154-9

摘要: Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ. [J]. Front. Environ. Sci. Eng., 2016, 10(3): 513-521.Yongtao LV,Xuan CHEN,Lei WANG,Kai JU,Xiaoqiang CHEN,Rui MIAO,Xudong WANG. [J]. Front. Environ. Sci. Eng., 2016, 10(2): 390-398.Zulkifly JEMAAT,Josep Anton TORA,Albert BARTROLI,Julián CARRERA,Julio PEREZ. [J]. Front. Environ. Sci. Eng., 2015, 9(3): 528-533.Wei LI, Xiaowen DING, Min LIU, Yuewen GUO, Lei LIU. [J]. Front Envir Sci Eng, 2012, 6(6): 892-900.Guochen ZHENG, Jianzheng LI, Feng ZHAO, Liguo ZHANG, Li WEI, Qiaoying BAN, Yongsheng ZHAO. [J]. Front Envir Sci Eng, 2012, 6(1): 125-130.Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN. [J]. Front Envir Sci Eng Chin, 2011, 5(4): 519-525.Daijun ZHANG, Cui BAI, Ting TANG, Qing YANG. [J]. Front Envir Sci Eng Chin, 2011, 5(2): 291-297.Yanhui ZHAN, Jianwei LIN, Yanling QIU, Naiyun GAO, Zhiliang ZHU. [J]. Front Envir Sci Eng Chin, 2011, 5(1): 65-75.Rongchang WANG, Xinmin ZHAN, Yalei ZHANG, Jianfu ZHAO. [J]. Front Envir Sci Eng Chin, 2011, 5(1): 48-56.Bo WANG, Wei WAN, Jianlong WANG, . [J]. Front.Environ.Sci.Eng., 2009, 3(4): 380-386.GUO Jinsong, YANG Guohong, FANG Fang, QIN Yu. [J]. Front.Environ.Sci.Eng., 2008, 2(4): 439-445.GENG Bing, ZHU Yanfang, JIN Zhaohui, LI Tielong, KANG Haiyan, WANG Shuaima. [J]. Front.Environ.Sci.Eng., 2007, 1(3): 357-361.WU Deyi, HU Zhanbo, WANG Xinze, HE Shengbing, KONG Hainan. [J]. Front.Environ.Sci.Eng., 2007, 1(2): 213-220.XU Zhengyong, YANG Zhaohui, ZENG Guangming, XIAO Yong, DENG Jiuhua. [J]. Front.Environ.Sci.Eng., 2007, 1(1): 43-48.

关键词: ammonium     anoxygenic phototrophic bacterium     biohydrogen     glutamine auxotrophic     tofu wastewater    

基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究 Article

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

《工程(英文)》 2022年 第9卷 第2期   页码 61-66 doi: 10.1016/j.eng.2021.05.012

摘要:

利用荧光原位杂交(FISH)和克隆文库构建技术,研究了厌氧污泥床反应器处理含酚废水时所收获的颗粒的微生物群落结构。研究发现,互营杆菌(Syntrophorhabdaceae)和隐杆厌氧菌(Cryptanaerobacter)的克隆是苯酚降解的主要原因。为了准确地对隐杆厌氧菌的克隆进行分类归属,需要利用近全长的16S核糖体RNA(rRNA)基因序列进行进化分析。设计了三种寡核苷酸探针用于检测以下三个分类群:互营杆菌、隐杆厌氧菌和互养菌。对厌氧颗粒薄片的FISH 分析显示细菌和古菌呈随机分布。然而,互营杆菌、隐杆厌氧菌和互养菌分布明确。在颗粒细胞的外层发现了隐杆厌氧菌和互养菌,两者关系密切,而互营杆菌则位于颗粒细胞的深层。细菌之间的代谢联系和对底物的亲和力很可能是造成这种特殊分布的原因。研究人员观察到颗粒污泥中苯酚的降解主要通过以下方式进行:首先,隐杆厌氧菌将苯酚转化为苯甲酸盐,然后被互养菌降解为乙酸盐。苯酚的这种互饲降解发生在颗粒表面附近,此处苯酚浓度较高。在苯酚浓度较低的颗粒深部,互营杆菌将苯酚降解为乙酸盐。结果表明,互营杆菌不太可能产生苯甲酸盐,并将其作为中间产物来喂养邻近的生物,这一点与以往研究所提出的理论相矛盾。

关键词: 隐杆厌氧菌(Cryptanaerobacter    荧光原位杂交     苯酚厌氧降解     互营杆菌(Syntrophorhabdaceae)     互养菌(Syntrophus    升流式厌氧污泥床(UASB)颗粒污泥    

标题 作者 时间 类型 操作

Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the

Bingjie Xu, Gang Xue, Xing Yang

期刊论文

Characterization of a phenanthrene-degrading methanogenic community

Quanhui Ye, Chengyue Liang, Chongyang Wang, Yun Wang, Hui Wang

期刊论文

A heterotrophic nitrification-aerobic denitrification bacterium TJPU05 suitable for nitrogen removal

期刊论文

Studies on culture condition of new marine bacterium sp. SY01

LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua

期刊论文

LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

期刊论文

Pollution and biodegradation of hexabromocyclododecanes: A review

Ling Huang, Syed Bilal Shah, Haiyang Hu, Ping Xu, Hongzhi Tang

期刊论文

Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

Longtao ZHANG, Bo JIANG, Wanmeng MU, Tao ZHANG,

期刊论文

Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter sphaeroides AR-3

Guanghong ZHENG, Zhuhui KANG, Yifan QIAN, Lei WANG,

期刊论文

基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

期刊论文